Composite

Part:BBa_K3939887

Designed by: Haiyang Liu   Group: iGEM21_Tianjin   (2021-09-30)


Cas9 gene regulated by tetR

The product of tetR gene expression binds to tetO to hinder the transcription of Cas9 protein. When anhydrous tetracycline is added, the inhibition can be released

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1901
    Illegal BglII site found at 2838
    Illegal BamHI site found at 3656
    Illegal XhoI site found at 5194
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 470
    Illegal NgoMIV site found at 4390
    Illegal AgeI site found at 1259
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None